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- W2374301203 abstract "This study was to analyze the expressions of Bmp8a and Bmp8b in cultured mouse granulosa cells,for further understanding of the mechanism of follicular development.Twenty-one to twenty-three day-old female mice were hypodermically injected with 10IU of PMSG.After 48 hours,the bilateral ovaries were removed and the granulosa cells were collected by piercing the ovarian follicles.The granulosa cells were seeded in cell culture plates in DMEM/F12 supplemented with 10% FBS.After 5 days,the cells were collected from the wells and total RNA was extracted.The expressions of Bmp8a and Bmp8b were analyzed by RT-PCR and confirmed by sequencing.RT-PCR sequencing results showed that in vitro cultured mouse granulosa cells generated BMP-8a transcript variant 2 and BMP-8b mRNA.And BMP-8a transcript variant 1 mRNA was found to be absent.Our results showed that in vitro cultured mouse granulosa cells could express BMP-8a transcript variant 2 and Bmp8b." @default.
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- W2374301203 date "2013-01-01" @default.
- W2374301203 modified "2023-09-24" @default.
- W2374301203 title "Expressions of Bmp8a and Bmp8b in cultured mouse granulosa cells." @default.
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