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- W2374305466 abstract "Fluorescence lifetime is dependent on the microenvironment of the fluorophore and its measurement and imaging can provide functional information about the sample. The principle, methods, current developments and biomedical applications of fluorescence lifetime imaging (FLIM) were discussed. A novel 5-D fluorescence microscopy that was the combination of a picosecond streak camera, a gradient-index microlens array, a prism spectrophotometer, a femtosecond Ti: Sapphire laser and a fluorescence microscope is presented. The system is versatile with multispectral capability, high temporal and spatial resolution and can be used to make 3-dimensitional multiphoton fluorescence intensity, spectrally resolved intensity and lifetime measurements with a single detector. Potential applications of this system include multiwell imaging, tissue discrimination, intracellular physiology and fluorescence resonance energy transfer imaging." @default.
- W2374305466 created "2016-06-24" @default.
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- W2374305466 date "2005-01-01" @default.
- W2374305466 modified "2023-09-26" @default.
- W2374305466 title "Fluorescence lifetime imaging and its biomedical applications" @default.
- W2374305466 hasPublicationYear "2005" @default.
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