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- W2374351659 abstract "AIM In order to understand the multiple functions of DNA-dependent protein kinase catalytic subunits(DNA-PKcs), its interacting proteins were identified. METHODS The co-immunoprecipitation (Co-IP), sodium dodecyl sulfate polyacry amide gel electrophoresis (SDS-PAGE), peptide mass fingerprint and Western blot analysis were used to isolate and identify the interacting proteins of DNA-PKcs. RESULTS The co-immunoprecipitation complex of DNA-PKcs antibody from HeLa cells were firstly obtained. Cyclin T2, a subunit of transcription elongation factor P-TEFb, was identified as a component of this interacting complex. The interaction between DNA-PKcs and cyclin T2 was further confirmed by Western blot analysis. CONCLUSION This is the first evidence demonstrating the interaction between DNA-PKcs and cyclin T2, which implies that DNA-PKcs could be involved in the process of gene transcription regulation or gene transcription coupled repair." @default.
- W2374351659 created "2016-06-24" @default.
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- W2374351659 date "2005-01-01" @default.
- W2374351659 modified "2023-09-25" @default.
- W2374351659 title "Identification of interaction between DNA-dependent protein kinase catalytic subunits and cyclin T2" @default.
- W2374351659 hasPublicationYear "2005" @default.
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