Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374356466> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2374356466 abstract "AIM: To isolate, culture and identify in vitro of dendritic cells (DCs) from human peripheral blood, and observe the function of antigen presentation. METHODS: The experiment was done from May 2005 to November 2006 in Biotherapy Laboratory Tumor Center, Nanfang Hospital, Southern Medical University. Peripheral blood monocytes were isolated from the healthy volunteers (who had known the facts), and then cultured for 5 hours. The adherent cells were washed and then cultured with RPMI1640 medium containing 10% AB serum, granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cellular morphous and phenotypes were examined, respectively at days 1, 3 and 6, and the cellular purity and yield were also detected. Peripheral blood was obtained from different healthy volunteers (who had known the facts). The blood was isolated with lymphocyte separating-medium, and non-adherent cell were cultured with 1640 medium containing 10% AB serum and interleukin-2 (IL-2). At the sixth day, the allogeneic T lymphocytes were harvested. The DCs were divided into two groups: one group was cultured with regular process for 6 days; another group was cultured with Melanoma Associated Antigen Gene A3 (MAGE-A3) peptide on the 5th day. After another 24 hours, 1×104,5×103,2×103,1×103 /per well of DCs were added in ultraviolet-treated 96-well plate. The auto-lymphocytes were used as control, and there were 3 sub-wells in each well, and 1×105 lymphocytes were added in each well to evaluate the ability of DCs stimulating lymphocytes proliferation. RESULTS: ①A large number of DCs with high purity (90.81%) were generated culturing for six days in vitro. DCs expressed highly 21.8%CD1a, 99.0%HLA-DR, 63.4%CD80, 18.9%CD83 and 80.6%CD86. ②Lymphocyte proliferation was induced by mixing allogeneic lymphocytes with variant proportional DCs cultured for 6 days. The ability of stimulating lymphocyte proliferation was significantly strengthened by various proportional DCs with MAGE-A3 peptide than corresponding proportional DCs without MAGE-A3 peptide. The most powerful effectiveness was developed with higher density of DCs, which could intensively stimulating allogeneic lymphocyte proliferation in mixed lymphocyte reaction. CONCLUSION: A group of dendritic cells, which highly express CD83, CD86 and HLA-DR molecules, and stimulate strongly allogeneic T lymphocyte proliferation in vitro, are obtained." @default.
- W2374356466 created "2016-06-24" @default.
- W2374356466 creator A5043760296 @default.
- W2374356466 date "2007-01-01" @default.
- W2374356466 modified "2023-09-23" @default.
- W2374356466 title "Isolation and identification of dendritic cells from human peripheral blood" @default.
- W2374356466 hasPublicationYear "2007" @default.
- W2374356466 type Work @default.
- W2374356466 sameAs 2374356466 @default.
- W2374356466 citedByCount "0" @default.
- W2374356466 crossrefType "journal-article" @default.
- W2374356466 hasAuthorship W2374356466A5043760296 @default.
- W2374356466 hasConcept C147483822 @default.
- W2374356466 hasConcept C153911025 @default.
- W2374356466 hasConcept C16685009 @default.
- W2374356466 hasConcept C202751555 @default.
- W2374356466 hasConcept C203014093 @default.
- W2374356466 hasConcept C2777371288 @default.
- W2374356466 hasConcept C2777478134 @default.
- W2374356466 hasConcept C2777761686 @default.
- W2374356466 hasConcept C2778690821 @default.
- W2374356466 hasConcept C3017910909 @default.
- W2374356466 hasConcept C55493867 @default.
- W2374356466 hasConcept C71924100 @default.
- W2374356466 hasConcept C86803240 @default.
- W2374356466 hasConcept C8891405 @default.
- W2374356466 hasConceptScore W2374356466C147483822 @default.
- W2374356466 hasConceptScore W2374356466C153911025 @default.
- W2374356466 hasConceptScore W2374356466C16685009 @default.
- W2374356466 hasConceptScore W2374356466C202751555 @default.
- W2374356466 hasConceptScore W2374356466C203014093 @default.
- W2374356466 hasConceptScore W2374356466C2777371288 @default.
- W2374356466 hasConceptScore W2374356466C2777478134 @default.
- W2374356466 hasConceptScore W2374356466C2777761686 @default.
- W2374356466 hasConceptScore W2374356466C2778690821 @default.
- W2374356466 hasConceptScore W2374356466C3017910909 @default.
- W2374356466 hasConceptScore W2374356466C55493867 @default.
- W2374356466 hasConceptScore W2374356466C71924100 @default.
- W2374356466 hasConceptScore W2374356466C86803240 @default.
- W2374356466 hasConceptScore W2374356466C8891405 @default.
- W2374356466 hasLocation W23743564661 @default.
- W2374356466 hasOpenAccess W2374356466 @default.
- W2374356466 hasPrimaryLocation W23743564661 @default.
- W2374356466 hasRelatedWork W1573432998 @default.
- W2374356466 hasRelatedWork W163471667 @default.
- W2374356466 hasRelatedWork W2139487123 @default.
- W2374356466 hasRelatedWork W2173600479 @default.
- W2374356466 hasRelatedWork W2348210487 @default.
- W2374356466 hasRelatedWork W2351140116 @default.
- W2374356466 hasRelatedWork W2360484154 @default.
- W2374356466 hasRelatedWork W2365811996 @default.
- W2374356466 hasRelatedWork W2367221596 @default.
- W2374356466 hasRelatedWork W2370288037 @default.
- W2374356466 hasRelatedWork W2371569175 @default.
- W2374356466 hasRelatedWork W2383576166 @default.
- W2374356466 hasRelatedWork W2385330004 @default.
- W2374356466 hasRelatedWork W2386043520 @default.
- W2374356466 hasRelatedWork W2390747645 @default.
- W2374356466 hasRelatedWork W2393591281 @default.
- W2374356466 hasRelatedWork W2424765226 @default.
- W2374356466 hasRelatedWork W2466205390 @default.
- W2374356466 hasRelatedWork W2945156593 @default.
- W2374356466 hasRelatedWork W2982613007 @default.
- W2374356466 isParatext "false" @default.
- W2374356466 isRetracted "false" @default.
- W2374356466 magId "2374356466" @default.
- W2374356466 workType "article" @default.