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- W2374385497 abstract "A simple and sensitive Reverse Transcription Loop-mediated Isothermal Amplification(RT-LAMP) method was established to detect Peanut Stripe Virus(PStV).A set of four specific primers were designed based on PStV coat protein gene for RT-LAMP assay.Amplification products were detected by SYBR green I staining and agarose gel electrophoresis.The specificity and sensitivity of the RT-LAMP assay were tested.The results showed that the RT-LAMP assay was rapid,specific and sensitive.The RT-LAMP reaction could be finished within 1 hour,and it was 10 times more sensitive than RT-PCR.The RT-LAMP assay reported here will become a useful method for rapid and reliable detection of PStV." @default.
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- W2374385497 date "2011-01-01" @default.
- W2374385497 modified "2023-09-23" @default.
- W2374385497 title "Detection of Peanut stripe virus by the reverse transcription loop-mediated isothermal amplification method" @default.
- W2374385497 hasPublicationYear "2011" @default.
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