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- W2374415926 abstract "Objective To explore differentiation of mesenchymal stem cells(MSCs) derived from bone marrow into target phenotype under hypoxia has been proposed as a potential therapeutic approach for related diseases.Methods In this study,we genetically modified MSCs with an anti-apoptotic GFP-Bcl-2 gene and evaluated cell survival and cell cycle under hypoxia in vitro.Rat bone marrow MSCs were transfected with lentiviral vectors over-expressing the GFP-Bcl-2 gene(GFP-Bcl-2-MSCs).The anti-apoptotic ability and cell cycle were assessed under hypoxic conditions.The cell cycle properties of the cultured cells(MSCs and GFP-Bcl-2-MSCs) were evaluated by using FACS.The expression of cyclinD1,cyclinE and PCNA was detected by using Western blotting.Results The Bcl-2 gene protected MSCs against apoptosis.In vitro,Bcl-2 over-expression reduced apoptosis of MSCs by 27% and enhanced cellular proliferation by more than 58% under hypoxic conditions.However,the MSCs and GFP-Bcl-2-MSCs had no significant difference in cell cycle distribution as well as the expression levels of cyclin D1,cyclinE and PCNA.Conclusion Anti-apoptotic gene-modified MSCs can validate the cell apoptosis.The mechanism under cell cycle needs further research." @default.
- W2374415926 created "2016-06-24" @default.
- W2374415926 creator A5026091430 @default.
- W2374415926 date "2013-01-01" @default.
- W2374415926 modified "2023-09-23" @default.
- W2374415926 title "Effect of modified GFP-Bcl-2 gene on mesenchymal stem cells cycle under hypoxic conditions in vitro." @default.
- W2374415926 hasPublicationYear "2013" @default.
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