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- W2374418612 abstract "OBJECTIVE A HPLC method with fluorescence detection for determination of 10-Hydroxycamptothecin in rabbit plasma.METHODS The chromatographic condition were as follows a Eclipse Discovery C_(18) column(4.6mm×150mm,5μm) was eluted with mobile phase citric acid buffer-acetonitrile-75nmKH2PO4(70:23:7,containing 1%triethylamine).the column temperature was 40℃,the flow rate was 1.0ml·min~(-1) with the fluorescence detection set at λex 363nm andλem 530nm.RESULTS The method was proved to be linear in the range of 13.7656～1957.4468ng·mL~(-1) with correlation coefficients 0.999343.the average recovery was 102.22%～108.16%,RSD of intra-day and inter-day were no more than 7.49% and 9.40%.The minimum detection limit was 5.2ng·mL~(-1).CONCLUSION The method is simple,good specificity,reproducibility,can used to investigate the pharma-cokinetics and determinate the concentration of 10-Hydroxycamptothecin." @default.
- W2374418612 created "2016-06-24" @default.
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- W2374418612 date "2006-01-01" @default.
- W2374418612 modified "2023-09-24" @default.
- W2374418612 title "Determination of 10-Hydroxycamptothecin by HPLC with Fluorescence Detection in Rabbit Plasma" @default.
- W2374418612 hasPublicationYear "2006" @default.
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