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- W2374430765 abstract "The method of Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) with TaqMan fluorescent probe was chosen to fast detect the amount of palm oil mixed in peanut oil.MT3-B gene of palm was selected as target gene to detect palm oil from peanut oil.The primers of MT3-B and TaqMan probe were designed,MT3-B gene reconstructed plasmid was built as absolute quantitative criteria for quantitative RT-PCR to establish standard curve.Peanut oil blended with 1%-40% concentration gradient palm oil was extracted DNA to test palm content by RT-PCR.The result showed that the correlation coefficient(R2) of standard curve with logarithmic linear regression analysis was 0.996.When the adulteration of palm oil in peanut oil reached 5% volume,MT3-B gene of 17.431copies per milliliter of mixed oil could be detected.The method showed good sensitivity,specificity and repeatability." @default.
- W2374430765 created "2016-06-24" @default.
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- W2374430765 date "2011-01-01" @default.
- W2374430765 modified "2023-09-24" @default.
- W2374430765 title "Determination of palm oil adulterated in peanut oil with the TaqMan probe-based RT-PCR method" @default.
- W2374430765 hasPublicationYear "2011" @default.
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