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- W2374438608 abstract "A pair of specific primers was designed and synthesized based on the nucleotide sequence of CMV coat protein genes reported before.The expected product(780 bp DNA fragment) was gained by one-step RT-PCR and two-step RT-PCR from the infected samples.Compared with two-step RT-PCR,one-step RT-PCR showed strong specificity,high sensitivity,simple procedure,economical and saving time,so it could meet short-term test.Thirty pepper samples with virus symptom collected from Shandong area were tested by one-step RT-PCR technique.The results showed that CMV was detected in 36.7% samples from the six sampling points.It indicated that CMV was one of the main virogenies of pepper virus diseases in Shandong Province." @default.
- W2374438608 created "2016-06-24" @default.
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- W2374438608 date "2009-01-01" @default.
- W2374438608 modified "2023-09-23" @default.
- W2374438608 title "Detection of CMV on Hot Pepper by RT-PCR" @default.
- W2374438608 hasPublicationYear "2009" @default.
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