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- W2374451795 abstract "Objective:The purpose of this research was to construct a lentivira vector containing murine IL-15 gene,and detect the expression of IL-15.Methods:The primers were designed according to the IL-15 gene sequence and the structure of lentiviral vector.Total RNA of mice splenocytes was extracted,and the coding sequence of IL-15 was amplified by RT-PCR.The PCR product was cloned and sequenced correctly.IL-15 gene was subcloned into the transfer plasmid of the lentivirus system,which was transfected together with the packaging plasmids into 293T cells.Western blot was used to detect the expression of IL-15 gene in the viral infected MEG cells.Results:PCR and DNA sequencing demonstrated that the lentivirus vector containing IL-15 gene was constructed successfully.MEG cells could be successfully infected by the recombinant lentivirus.The positive rate of infection was up to 98% detected by FACS.IL-15 protein could be expressed in the infected MEG cells analyzed by Western blot.Conclusion:The lentiviral vector containing murine IL-15 gene was constructed successfully which provides basis for future research on the role of IL-15 during immunological regulation.." @default.
- W2374451795 created "2016-06-24" @default.
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- W2374451795 date "2010-01-01" @default.
- W2374451795 modified "2023-09-25" @default.
- W2374451795 title "Construction and expression of murine IL-15 gene lentiviral vector" @default.
- W2374451795 hasPublicationYear "2010" @default.
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