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- W2374461844 abstract "A analytical method for simultaneous determination of adenosine triphosphate(ATP),adenosine diphosphate(ADP) and adenosine monophosphate(AMP) in fresh tobacco leaves was established by ultra high performance liquid chromatography(UPLC) with phenol extraction.ATP,ADP and AMP in phenol extraction were stable and difficult to be hydrolyzed with rate of change less than 10% within 2 hours.A better separation of ATP,ADP and AMP was achieved in 10 minutes by utilizing a gradient elution system of 6 ‰triethylamine(pH = 6.6) and methanol as mobile phase.The linear range was between 5~200 μg.mL-1 with adenosine concentration and peak area were highly correlated(r 0.9999).The detection limits for ATP,ADP and AMP were 0.5 μg.mL-1,0.4 μg.mL-1,0.3 μg.mL-1 respectively,and average recovery rate was 91.68 % ~ 110.79 % with RSD ranged between 1.53 %~3.67 %.This method can quickly and accurately detect ATP content in tobacco samples,thus providing important test indicators for the study of energy metabolism,especially tobacco CMS generation mechanism." @default.
- W2374461844 created "2016-06-24" @default.
- W2374461844 creator A5057769470 @default.
- W2374461844 date "2014-01-01" @default.
- W2374461844 modified "2023-09-23" @default.
- W2374461844 title "Determination of adenosine phosphate in tobacco leaf by UPLC with phenol-TEA pretreatment" @default.
- W2374461844 hasPublicationYear "2014" @default.
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