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- W2374466221 abstract "[Objective] To establish a method to isolate and cultivate the mesenchymal stem cells(MSCs) obtained from the rat bone marrow and identify them;To study the transfer efficiency of plasmid vector coding enhanced green fluorescence protein(EGFP) gene into MSCs by electroporation method.[Methods] Rat MSCs were separated and purified by gradient centrifugation with Ficoll-PaqueTMPlus.The cells were cultivated and then expanded by subculture successively.The morphology was observed with phasecontrast microscope.The immuno-phenotype of the MSCs were evaluated with immunocytochemical methods.The pIRES2-EGFP transferred MSCs by means of electroporation.Transfer efficiency were evaluated by fluorescent microscopy and flow cytometry technique.[Results] The cultured cells were spindle shaped and proliferated fast before the seventh generation;They uniformly expressed CD_(29),CD_(44),CD_(106),CD_(54),but not CD_(34)、 Laminin、CollagenⅡ.Flow cytometry analysis showed that 32.8%±3% of the cells were positive for EGFP at 48 hours after electroporation.[Conclusion] Rat MSCs can be efficiently separated by method of gradient centrifugation and successfully expanded in vitro.Transferring exogenous gene into MSCs can be achieved by electroporation." @default.
- W2374466221 created "2016-06-24" @default.
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- W2374466221 date "2007-01-01" @default.
- W2374466221 modified "2023-09-24" @default.
- W2374466221 title "Isolation and cultivation of Rat BMMSCs and transferring of EGFP" @default.
- W2374466221 hasPublicationYear "2007" @default.
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