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- W2374496240 abstract "The factors influencing SRAP analysis of lettuce(Lactuca sativa L.) including the concentration of Mg2+,dNTP,Taq DNA polymerase,primers,DNA template and annealing temperature in PCR were investigated.The optimum reaction system was as following: the 20μl reaction solution contains 50ng DNA,Mg2+ 2.0 mmol/L,dNTP 0.2 mmol/L,Taq DNA polymerase 1U,primer 0.75μmol/L.Reactions were carried out in two steps: the first 5 cycles consist of denature at 94℃,annealing at 35℃ and extension at 72℃ after pre-denature at 94℃ for 5min,the second 30 cycles include the same conditions for denature and extension as in the first step with the changed annealing temperature at 51℃.The newly established SRAP-PCR system for Lactuca sativa L.was fully reproducible with high reliability,easy isolation of bands,rich DNA polymorphism,which could be applied in genetic evaluation of lettuce germplasm and in breeding of molecular assisted selection." @default.
- W2374496240 created "2016-06-24" @default.
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- W2374496240 date "2008-01-01" @default.
- W2374496240 modified "2023-09-26" @default.
- W2374496240 title "Optimization for SRAP-PCR Reaction System in Lactuca sativa L." @default.
- W2374496240 hasPublicationYear "2008" @default.
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