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- W2374500418 abstract "【Objective】 The experiment was conducted to study cell apoptosis and possible mechanism of human hepatoma QGY-7703 cells induced by salidroside in vitro culture.【Method】 With human hepatoma QGY-7703 cells as study object in vitro culture,the QGY-7703 growth inhibition effect of salidroside was measured by methyl thiazolyl tetrazolium(MTT) method;The apoptosis effect of QGY-7703 was analyzed by AO/EB double fluorescent dye staining,agarose gel electrophoresis and flow cells analysis;The possible mechanism of QGY-7703 apoptosis induced by salidroside was studied by western blot.【Result】 The result of MTT method showed that salidroside of 0.01,0.1,1,10 and 100 μg/mL all inhibited the growth of QGY-7703 cells.The cell growth inhibition rate of QGY-7703 was 13.2%,21.9%,31.4%,46.8% and 60.9% respectively and the 50% inhibiting concentration(IC50) was 18.56 μg/mL.By AO/EB double fluorescent dye staining treated with 10 μg/mL salidroside for 48 h,individual cells presented bright green and jacinth.It showed that these cells apoptosised.The DNA in nucleus appeared regular rupture and formed DNA ladder treated with 10 μg/mL salidroside for 24,48,and 72 h in agarose gel electrophoresis experiment.The result of flow cells analysis showed that the apoptosis cells were 178 and 331 and the apoptosis rate was 1.8% and 3.5% respectively when added amount of 10 μg/mL salidroside was 100 μL and 200 μL,but the apoptosis cells were 105 and the apoptosis rate was only 1.1% in the control group(the volume of 10 μg/mL salidroside was 0 μL).Western blot showed that salidroside down-regulated the expression of Bcl-2 and PCNA proteins,but protein expressions of apoptosis activation gene Bax and tumor suppressor gene p53 were up-regulated.【Conclusion】 The salidroside could inhibit cell growth and induce cell apoptosis of OGY-7703 cells in vitro culture.The effect of apoptosis was related to the gene modulation of Bcl-2 family,p53,Bax and PCNA." @default.
- W2374500418 created "2016-06-24" @default.
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- W2374500418 date "2011-01-01" @default.
- W2374500418 modified "2023-09-23" @default.
- W2374500418 title "Study on cell apoptosis of human hepatoma QGY-7703 cell induced by salidroside in vitro culture" @default.
- W2374500418 hasPublicationYear "2011" @default.
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