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- W2374559384 abstract "Rice and wheat PAL have been isolated and purified to homogeneity by ammonium sulphate fractionation, DEAE-cellulose chromatography and affinity chromatography on L-Phe-Sepharose column. The purity was identifited by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulphate (SDS).Many properties of the two PALs are roughly similar, but there are some differences.Both PALs possess certain thermostability and rice PAL is more thermostable than wheat PAL. The optimum pH of the two PALs are both in the alkaline side, but the optimum pH of the PAL of rice is slightly higher than that of wheat, being 9.2 and 8.8, respectively. Both PALs were inhibited by sulfhydryl reagent, NEM. The final specific activities of rice and wheat PAL were 1350 and 454u (mg protien)~(-1) respectively.The K_m values for rice PAL has been shown to be 5.94×10~(-4)M, whereas the wheat PAL possess two K_m values — 3.1×10~(-4)M and 0.625×10~(-4)M.The results of amino acid analysis of PAL show that rice PAL contains less dicarboxylic amino acids and more neutral and basic amino acids than wheat PAL.The molecular weight of rice PAL subunit is 57,500 and that of wheat is 70,000 as assayed by SDS polyacrylamide gel electrophoresis. The subunit of either PAL migrated as a single band. Since the PALs consist of four similar subunits, the total enzyne molecular weights of rice and wheat PALs should be about 230,000 and 280,000 Dal., respectively." @default.
- W2374559384 created "2016-06-24" @default.
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- W2374559384 date "1985-01-01" @default.
- W2374559384 modified "2023-09-28" @default.
- W2374559384 title "STUDIES ON PLANT PHENYLALANINE AMMONIA-LYASE (PAL) VI. PURIFICATION AND SOME PROPERTIES OF PAL FROM ETIOLATED SEEDLINGS OF RICE (ORYZA SATIVA) AND WHEAT (TRITICUM AESTIVUM)" @default.
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