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- W2374560754 abstract "Objectives To observe the effect of the conditioned medium of VEC induced by ox-LDL on the growth of VSMC. Methods By using MTT staining, liquid cellular technique and the immune histochemistry method of the proliferous nucleus antigen respectively, the influence on the growth rate, cell cycle and PCNA expression of the cultured rat thoracic aorta smooth muscle cells caused by the conditioned medium leading to injury to VEC induced by ox-LDL were assayed. Results ox-LDL can injure the morphology of VEC in the form of reliance of the concentration, reduce the content of NO in the supernatant, and elevate the content of ET-l in the supernatant. The conditioned medium which is prepared with the end concentration 100ug/ml of ox-LDL and VEC for 24 hours can obviously promote the growth of VSMC, increase the expression of PCNA and Phase S of the cellular cycle. Conclusion When the vascular endothelial cells are injured by ox-LDL, the prepared conditioned medium is able to promote the proliferation of VSMC. This may be one of the reasons for the formation of atherosclerosis induced by ox-LDL." @default.
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- W2374560754 date "2004-01-01" @default.
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- W2374560754 title "The Effect of the Conditioned Medium of VEC Induced by ox-LDL on the Proliferation of VSMC" @default.
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