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- W2374597188 abstract "Objective To design a hammerhead ribozyme targeting HIF-1α mRNA and clone its gene into an expression vector for future use in the study of tumor gene therapy.Methods Using the software RNA structure, the secondary structure of HIF-1α mRNA was predicted and the cleavage site of ribozyme was decided. A hammerhead ribozyme targeting this site was designed. The DNA encoding the ribozyme was synthesized and cloned into the vector pSP72 and the sequence of the ribozyme gene was confirmed by endonuclease digestion and DNA sequencing.Result Triplet GUC at 72 of HIF-1α mRNA was chosen as the cleavage site of the ribozyme. The designed ribozyme comprised of 22 nt catalytic core and 16 nt flanking sequence. Computer-aided prediction suggested that this site was the proper targeting site. Endonuclease restriction and DNA sequencing confirmed the correct insertion of the ribozyme gene into the vector pSP72.Conclusion The present fundamental work of successful designing and cloning of an anti-HIF-1α mRNA hammerhead ribozyme has paved the way for further study on inhibiting tumor growth by cleaving HIF-1α mRNA with ribozyme." @default.
- W2374597188 created "2016-06-24" @default.
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- W2374597188 date "2004-01-01" @default.
- W2374597188 modified "2023-09-28" @default.
- W2374597188 title "Design of a hammerhead ribozyme targeting HIF-1α mRNA and cloning of its gene" @default.
- W2374597188 hasPublicationYear "2004" @default.
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