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- W2374608045 abstract "A pET30a-GFP recombinant plasmid was constructed by inserting GFP gene into pET30a vector, and was transformed into E. Coli. BL-21. After induced by IPTG, the fusion protein (His-GFP) was expressed mainly in the suspension. His-GFP was purified by Ni resins affinity chromatography. The molecular of purified His-GFP was about 32.4 kD as that of deduced from gene. The fluorescence wavelength of His-GFP is similar as that of wildness. The fluorescence characteristic of His-GFP is stable and His-GFP has stronger anti-fluorescence quenching than that of the regular fluorescence reagent (FITC). The GFP produced by this method could be used as edible pigment." @default.
- W2374608045 created "2016-06-24" @default.
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- W2374608045 date "2006-01-01" @default.
- W2374608045 modified "2023-09-25" @default.
- W2374608045 title "THE PROKARYOTIC EXPRESSION,PURIFICATION AND FLUORESCENCE ANALYSIS OF A GREEN FLUORESCENT PROTEIN (GFP)" @default.
- W2374608045 hasPublicationYear "2006" @default.
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