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- W2374612897 abstract "Objective To investigate the inhibitory effect of siRNA targeting casein kinase 2(CK2α)gene on the growth of HCT116cells and to clarify its mechanism.Methods CK2α-siRNA sequence was designed according to mRNA sequence of CK2α.The in vitro cultured HCT116cells were divided into normal control group(without transfection),negative control group(transfected with siRNA)and CK2α-siRNA group(transfected with CK2α-siRNA),and the HCT116cells were transfected with Lipofectamine 2000.The expression levels of CK2α,cyclin H,P53,and P21proteins in the HCT116cells were detected by Western blottting method,and the proliferation activities of the HCT116cells were detected by MTT method,and the cell cycle was measured by flow cytometry. Results Compared with negative control group,the expression levels of CK2α,and cyclin H proteins in CK2α-siRNA group were decreased(P0.01);the expression level of P53protein had no change dramatically(P0.05), and the expression level of P21protein was increased significantly(P0.01).Compared with negative control group,the survival rate in CK2α-siRNA group was decreased markedly 48and 72hafter transfection detected by MTT method(P0.01).Flow cytometry analysis showed the percent of the cells at G1 phase in CK2α-siRNA group was significantly higher than that in negative control group and the percent of the cells at S phase in CK2α-siRNA group was lower than that in negative control group(P0.01),and the cell cycle was arrested at G1phase. Conclusion siRNA targeting CK2αcan inhibit the proliferation of HCT116cells and induce the arrest of G1phase, which may be associated with inhibiting the expression of cyclin H and recovering the P53activity after silencing CK2α." @default.
- W2374612897 created "2016-06-24" @default.
- W2374612897 creator A5070538645 @default.
- W2374612897 date "2014-01-01" @default.
- W2374612897 modified "2023-09-22" @default.
- W2374612897 title "Inhibitory effect of siRNA targeting CK2α gene on growth of HCT116 cells and its mechanism" @default.
- W2374612897 hasPublicationYear "2014" @default.
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