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- W2374617903 abstract "The casein culture medium was used to isolate milk-clotting enzyme producing bacteria from soil of yak grazing district in Gannan County.Strain identification was carried out based on the morphology,physiology,biochemical test and 16S rDNA sequences analysis,and the property of the milk-clotting enzyme produced by the strain was studied.Six bacteria producing milk-clotting enzyme were screened out.Furthermore,one bacterium GN4.1 had the highest milk-clotting enzyme activity and the lowest protein hydrolyzed enzyme activity,identified as Bacillus amyloliquefaciens.The milk-clotting enzyme activity and the protein hydrolyzed enzyme activity of GN 4.1 was 1011.56SU/mL and 14.61U/mL after fermentation for 48h,respectively.The optimal reaction condition for milk-clotting enzyme was at 60℃,pH5.5.The activity was stable at pH ranging from 3.5 to 8.5 but totally lost after heating at 65℃ for 10min.The milk-clotting enzyme produced by strain GN 4.1 might have the potential applicatin in dairy industry." @default.
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- W2374617903 date "2012-01-01" @default.
- W2374617903 modified "2023-09-28" @default.
- W2374617903 title "Study on isolation,identification of Bacillus amyloliquefaciens producing chymosin and enzyme properties" @default.
- W2374617903 hasPublicationYear "2012" @default.
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