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- W2374664862 abstract "Objective: To research the expression of mucin-2(MUC2)after the overexpression of caudal type ho-meobox 2(CDX2)in umbilical cord mesenchymal stem cells(UC-MSC), explore the method of making MUC2 over-express in MSC by genetical modification,study the necessity that inducing UC-MSC differentiate into endodermalcell for overexpressing MUC2, and lay the foundation for studying UC-MSC differentiate into goblet cells in intesti-nal mucosa. Methods: The experiment was divided into 5 groups, they were single-transfection group(CDX2 wastransfected into UC-MSC), single-induction group(induce UC-MSC differentiate into endodermal cells), inductionand transfection group(CDX2 was transfected into UC-MSC after induction), single-induction group(just transfect-ing vector without CDX2) and control group(UC-MSC without any treatment). Single-induction group should betreated by transfection reagent in order to exclude the effect of transfection reagen. Finally, the mRNA of MUC2 and CDX2 were detected in each group. The endodermal cells was induced by recombinant human Activin A, re-combinant human Wnt3 a. Results: Green fluorescence can be seen by fluorescence microscope in single-transfec-tion group, induction and transfection group and single-induction group after 48 h. GATA4 and Sox17 increase by27 fold and 54 fold respectively, which both were marker of endoderm until the 5th day when UC-MSC was in-duced in single-transfection group and induction and transfection group. The mRNA of five groups was detected by RT-PCR, the result showed that CDX2 increase by 113 fold, MUC2 increase by 30 fold in single-transfectiongroup; CDX2 increase by 196 fold, MUC2 increase by 98 fold in induction and transfection group. The expressionof CDX2 and MUC2 didn't change significantly in the other three groups. Conclusion: Overexpression of CDX2 can increase the expression of MUC2 that is marker of goblet cell in UC-MSC. The expression of MUC2 can beincreased if UC-MSC is induced to endodermal cells in advace. So it is possible to induce UC-MSC differentiateinto goblet cells by overepression of CDX2 and induction to endodermal cells can make it easier." @default.
- W2374664862 created "2016-06-24" @default.
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- W2374664862 date "2014-01-01" @default.
- W2374664862 modified "2023-09-25" @default.
- W2374664862 title "Feasibility Study of Umbilical Cord Mesenchymal Stem Cells Modified by CDX2 Differentiating into Goblet Cells" @default.
- W2374664862 hasPublicationYear "2014" @default.
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