Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374667097> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2374667097 abstract "Objective To establish the genetically engineered neural stem cells(NSCs) for further biomedical applications. Methods ① After isolated from fetal rat telencephalon and identified with nestin immunocytochemical staining, NSCs were induced to differentiate. The differentiated cells were detected respectively with neurofinament (NF 200) and glial fibrillary acidic protein(GFAP) immunocytochemical staining. ② Recombinant brain-derived neurotrophic factor (BDNF) adenovirus was used to infect the obtained cells and the results were identified with in situ hybridization. Results ① Nest like clusters of neural stem cells were obtained in the suspension and the cells could be differentiated into neurons and astrocytes . ② About 80%-90% of NSCs were infected by recombinant BDNF adenovirus, which retaining the main characteristics of NSCs after 12 passages of culture. Conclusion The cells from fetal rat telencephalon possess multipotency and self renew ability and is believed to be BSCs of the central nervous system.Since they retain their characteristics and differentiation ability after passages and genetic engineering, they can be applied widely in basic research and as grafts in neural transplantation." @default.
- W2374667097 created "2016-06-24" @default.
- W2374667097 creator A5086756275 @default.
- W2374667097 date "2001-01-01" @default.
- W2374667097 modified "2023-09-25" @default.
- W2374667097 title "Isolation, culture and transfection of neural stem cells from fetal rat telencephalon" @default.
- W2374667097 hasPublicationYear "2001" @default.
- W2374667097 type Work @default.
- W2374667097 sameAs 2374667097 @default.
- W2374667097 citedByCount "0" @default.
- W2374667097 crossrefType "journal-article" @default.
- W2374667097 hasAuthorship W2374667097A5086756275 @default.
- W2374667097 hasConcept C104317684 @default.
- W2374667097 hasConcept C136834591 @default.
- W2374667097 hasConcept C145103041 @default.
- W2374667097 hasConcept C153911025 @default.
- W2374667097 hasConcept C160539049 @default.
- W2374667097 hasConcept C162746124 @default.
- W2374667097 hasConcept C169760540 @default.
- W2374667097 hasConcept C170493617 @default.
- W2374667097 hasConcept C203014093 @default.
- W2374667097 hasConcept C204232928 @default.
- W2374667097 hasConcept C20875687 @default.
- W2374667097 hasConcept C2777738006 @default.
- W2374667097 hasConcept C2780495277 @default.
- W2374667097 hasConcept C28328180 @default.
- W2374667097 hasConcept C529278444 @default.
- W2374667097 hasConcept C533869091 @default.
- W2374667097 hasConcept C54355233 @default.
- W2374667097 hasConcept C86803240 @default.
- W2374667097 hasConcept C95444343 @default.
- W2374667097 hasConceptScore W2374667097C104317684 @default.
- W2374667097 hasConceptScore W2374667097C136834591 @default.
- W2374667097 hasConceptScore W2374667097C145103041 @default.
- W2374667097 hasConceptScore W2374667097C153911025 @default.
- W2374667097 hasConceptScore W2374667097C160539049 @default.
- W2374667097 hasConceptScore W2374667097C162746124 @default.
- W2374667097 hasConceptScore W2374667097C169760540 @default.
- W2374667097 hasConceptScore W2374667097C170493617 @default.
- W2374667097 hasConceptScore W2374667097C203014093 @default.
- W2374667097 hasConceptScore W2374667097C204232928 @default.
- W2374667097 hasConceptScore W2374667097C20875687 @default.
- W2374667097 hasConceptScore W2374667097C2777738006 @default.
- W2374667097 hasConceptScore W2374667097C2780495277 @default.
- W2374667097 hasConceptScore W2374667097C28328180 @default.
- W2374667097 hasConceptScore W2374667097C529278444 @default.
- W2374667097 hasConceptScore W2374667097C533869091 @default.
- W2374667097 hasConceptScore W2374667097C54355233 @default.
- W2374667097 hasConceptScore W2374667097C86803240 @default.
- W2374667097 hasConceptScore W2374667097C95444343 @default.
- W2374667097 hasLocation W23746670971 @default.
- W2374667097 hasOpenAccess W2374667097 @default.
- W2374667097 hasPrimaryLocation W23746670971 @default.
- W2374667097 hasRelatedWork W2038368018 @default.
- W2374667097 hasRelatedWork W2103378082 @default.
- W2374667097 hasRelatedWork W2105013029 @default.
- W2374667097 hasRelatedWork W2186003286 @default.
- W2374667097 hasRelatedWork W2355534764 @default.
- W2374667097 hasRelatedWork W2357665232 @default.
- W2374667097 hasRelatedWork W2371605668 @default.
- W2374667097 hasRelatedWork W2394385968 @default.
- W2374667097 hasRelatedWork W2400391304 @default.
- W2374667097 hasRelatedWork W2441203210 @default.
- W2374667097 hasRelatedWork W2557780209 @default.
- W2374667097 hasRelatedWork W2901424192 @default.
- W2374667097 hasRelatedWork W3010981644 @default.
- W2374667097 hasRelatedWork W3025781681 @default.
- W2374667097 hasRelatedWork W3028630455 @default.
- W2374667097 hasRelatedWork W3028673204 @default.
- W2374667097 hasRelatedWork W3031268908 @default.
- W2374667097 hasRelatedWork W3148301540 @default.
- W2374667097 hasRelatedWork W3151266371 @default.
- W2374667097 hasRelatedWork W3028998041 @default.
- W2374667097 isParatext "false" @default.
- W2374667097 isRetracted "false" @default.
- W2374667097 magId "2374667097" @default.
- W2374667097 workType "article" @default.