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- W2374686304 abstract "Objective To investigate the effects of bisphenol A(BPA) on spermatogenic cell apoptosis of offspring mice prior and postnatal to birth,and explore the possible molecule mechanism of BPA treatment.Methods ICR pregnant mice(0 day) were randomly divided into control group,solvent group,10 nmol/L,100 nmol/L,and 1000 nmol/L BPA groups,respectively.Pregnant mice of BPA groups were feed with BPA treated water.Testicular tissues of male offspring mice were taken out for evaluating testicular index at the six weeks after the birth.Ultrastructure of testicular tissue was observed under electron microscope,cell apoptosis was measured by Hoechst dyeing,and caspase-3 expression was detected by immunohistochemical method.Results Compared with control and solvent groups,testicular index decreased in all BPA groups.Under electron microscopy,mitochondria vacuole and rough endoplasmic reticulum expansion appeared in leydig's cells,spermatagonial cells and support cells of 100 nmol/L and 1000 nmol/L BPA groups.Hoechst dyeing showed that cell apoptosis was more significant in 100 nmol/L and 1000 nmol/L BPA groups than that in control and 10 nmol/L BPA group.Immunohistochemical results showed the level of caspase-3 was more high in 100 nmol/L and 1000 nmol/L BPA groups,which mainly appeared in spermatagonial cells,leydig's cells and the leydig's cells.Conclusions Bisphenol A affect histological structure of the testicles through inducing apoptosis of sperm cells and stromal cell." @default.
- W2374686304 created "2016-06-24" @default.
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- W2374686304 date "2012-01-01" @default.
- W2374686304 modified "2023-09-27" @default.
- W2374686304 title "Effects of bisphenol A on spermatogenic cell apoptosis of male offspring mice prior and postnatal to birth" @default.
- W2374686304 hasPublicationYear "2012" @default.
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