Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374720607> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2374720607 abstract "Objective To develop a method of Fluorescent Quantitative and Qualitative Loop-mediated Isothermal Amplification(LAMP) to Bacillus anthracis rapidly,specifically,sensitively and simply suited for used in the primary public health agency.Methods According to conserved nucleotide of Bacillus anthracis and principle of LAMP,a set of LAMP primers was designed and a LAMP and a real-time LAMP reaction system was set up.The specificity,sensitivity and repeatability of the method was evaluated.In addition,the linearity of the real-time LAMP between initial template copies Log value and reaction time was evaluated.Results The optimal assay showed that it was no cross-reaction with the other bacteria belong to Bacillus cereus group,and the threshold values of detection for Bacillus anthracis was 103copies per reaction,10 times more sensitive than PCR,and The Coefficient of Variance between tests was less than 5%。The kinetics curves showed a good linearity between initial template copies Log value and reaction time,which correlation coefficient was 0.9891.Conclusion These findings demonstrate that the fluorescent quantitative and qualitative LAMP has the potential clinical application for detection and differentiation of Bacillus anthracis in the primary hospital,inspection and quarantine institution for its sensitive,specific and simple feature." @default.
- W2374720607 created "2016-06-24" @default.
- W2374720607 creator A5030014001 @default.
- W2374720607 date "2013-01-01" @default.
- W2374720607 modified "2023-09-24" @default.
- W2374720607 title "Study on detection of Bacillus anthracis and its quantitate based on Fluorescent Quantitative Loop-mediated Isothermal Amplification" @default.
- W2374720607 hasPublicationYear "2013" @default.
- W2374720607 type Work @default.
- W2374720607 sameAs 2374720607 @default.
- W2374720607 citedByCount "0" @default.
- W2374720607 crossrefType "journal-article" @default.
- W2374720607 hasAuthorship W2374720607A5030014001 @default.
- W2374720607 hasConcept C120665830 @default.
- W2374720607 hasConcept C121332964 @default.
- W2374720607 hasConcept C154020017 @default.
- W2374720607 hasConcept C185592680 @default.
- W2374720607 hasConcept C186060115 @default.
- W2374720607 hasConcept C2777118263 @default.
- W2374720607 hasConcept C2780239966 @default.
- W2374720607 hasConcept C43617362 @default.
- W2374720607 hasConcept C523546767 @default.
- W2374720607 hasConcept C54355233 @default.
- W2374720607 hasConcept C552990157 @default.
- W2374720607 hasConcept C55493867 @default.
- W2374720607 hasConcept C60635243 @default.
- W2374720607 hasConcept C86803240 @default.
- W2374720607 hasConcept C89423630 @default.
- W2374720607 hasConcept C91881484 @default.
- W2374720607 hasConceptScore W2374720607C120665830 @default.
- W2374720607 hasConceptScore W2374720607C121332964 @default.
- W2374720607 hasConceptScore W2374720607C154020017 @default.
- W2374720607 hasConceptScore W2374720607C185592680 @default.
- W2374720607 hasConceptScore W2374720607C186060115 @default.
- W2374720607 hasConceptScore W2374720607C2777118263 @default.
- W2374720607 hasConceptScore W2374720607C2780239966 @default.
- W2374720607 hasConceptScore W2374720607C43617362 @default.
- W2374720607 hasConceptScore W2374720607C523546767 @default.
- W2374720607 hasConceptScore W2374720607C54355233 @default.
- W2374720607 hasConceptScore W2374720607C552990157 @default.
- W2374720607 hasConceptScore W2374720607C55493867 @default.
- W2374720607 hasConceptScore W2374720607C60635243 @default.
- W2374720607 hasConceptScore W2374720607C86803240 @default.
- W2374720607 hasConceptScore W2374720607C89423630 @default.
- W2374720607 hasConceptScore W2374720607C91881484 @default.
- W2374720607 hasLocation W23747206071 @default.
- W2374720607 hasOpenAccess W2374720607 @default.
- W2374720607 hasPrimaryLocation W23747206071 @default.
- W2374720607 hasRelatedWork W1545229485 @default.
- W2374720607 hasRelatedWork W2012187920 @default.
- W2374720607 hasRelatedWork W2048803459 @default.
- W2374720607 hasRelatedWork W2053166699 @default.
- W2374720607 hasRelatedWork W2137093358 @default.
- W2374720607 hasRelatedWork W2344732879 @default.
- W2374720607 hasRelatedWork W2348021573 @default.
- W2374720607 hasRelatedWork W2364763336 @default.
- W2374720607 hasRelatedWork W2366338016 @default.
- W2374720607 hasRelatedWork W2367141931 @default.
- W2374720607 hasRelatedWork W2378351399 @default.
- W2374720607 hasRelatedWork W2383065560 @default.
- W2374720607 hasRelatedWork W2383716802 @default.
- W2374720607 hasRelatedWork W2386805518 @default.
- W2374720607 hasRelatedWork W2387800053 @default.
- W2374720607 hasRelatedWork W2392327862 @default.
- W2374720607 hasRelatedWork W2805581868 @default.
- W2374720607 hasRelatedWork W3032189136 @default.
- W2374720607 hasRelatedWork W3157058455 @default.
- W2374720607 hasRelatedWork W174784977 @default.
- W2374720607 isParatext "false" @default.
- W2374720607 isRetracted "false" @default.
- W2374720607 magId "2374720607" @default.
- W2374720607 workType "article" @default.