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- W2374732934 abstract "Objective To investigate the effect of Raman spectroscopy for isoprenaline hydrochloride(Iso)-induced liver injury after myocardial fibrosis of ischemia in the rats,and to provide the basis for the application of Raman spectroscopy.Methods 20 Wistar rats were randomly divided into control group,Iso-4hgroup,Iso-24 hgroup,and Iso-1week group(n= 5).Except the normal group,the rats in other groups were subcutaneously injected with Iso.The pathological changes of heart and liver tissues were analyzed by HE staining.The absorption spectra of proteins in the liver tissue was analyzed by UV and Raman spectroscopy.Results The pathological observation results showed that the cardiomyocytes of the rats in control group arranged in neat rows with clear stripes,obvious nucleus and no cell swelling;the cardiomyocytes of the rats in Iso-24 hgroup appeared the more necrosis limting under endocardium;the myocardium of the rats in Iso-1week group appeared the multiple scattered necrosis with fibroblasts and collagen fiber.The hepatic lobules of the rats in control group had clear boundaries without necrosis;the hepatic lobules of the rats in Iso groups had focal necrosis,and the liver cells appeared watery degeneration;with the prolongation of the Iso injection time,the degree of liver lesion was serious.The UV spectroscopy showed that the myocardial protein spectrum of the rats in Iso-24 hgroup moved 1unit to the left or the right,and the spectrum in Iso-1 week group became nromal compared with control group;the liver protein spectrum of the rats in Iso-24 hgroup moved 2units,and the spectrum in Iso-1week gradually stabilized compared with control group.The Raman spectrocopy showed that the absorption peak of liver tissue in Iso-24 hgroup appeared at 1 590 nm,but it wasn't found in Iso-1 week group.Conclusion Liver damage is found when Iso induces myocardium injury,and Raman spectroscopy is a more sensitive detection method for liver damage." @default.
- W2374732934 created "2016-06-24" @default.
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- W2374732934 date "2015-01-01" @default.
- W2374732934 modified "2023-09-23" @default.
- W2374732934 title "Analysis of Raman spectroscopy for Iso-induced liver injury after myocardial fibrosis of ischemia in rats" @default.
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