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- W2374733877 abstract "可回复性永生化的基本原理是:先将永生化基因SV40T(猿猴病毒40大T抗原)导入原代细胞以获得永生化细胞株,使其获得体外增殖能力,然后再以特异性位点重组技术切除SV40T基因,使细胞株回复到永生化前的状态,这一技术已被用于多种哺乳动物细胞的永生化[1,2].本研究在以往研究的基础上,构建了包括雌激素受体(ER)与重组酶Cre的融合基因Cre-ER、永生化基因SV40T、加强型绿色荧光蛋白(EGFP)与胸苷激酶(TK)的融合基因EGFp-TK以及重组位点loxP序列的可回复性永生化逆转录病毒载体,旨在为肝细胞或其他细胞的可回复永生化奠定基础。" @default.
- W2374733877 created "2016-06-24" @default.
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- W2374733877 date "2006-03-01" @default.
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- W2374733877 title "[Construction and reversion of pCTGTKlox for reversible hepatocyte immortalization]." @default.
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