Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374738363> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2374738363 abstract "Objective: To investigate pp-10,a monomer purified from Paris Polyphylla,induced apoptosis of human gastric carcinoma BGC-823 cells and the possible mechanism behind this effect. Methods: The effects of pp-10 on the proliferation of BGC-823 Cells were evaluated by the MTT assays and colony formation assays. The cellular morphology changes of BGC-823 cells were observed by fluorescence microscopy after Hoechst 33342 staining. Cell apoptosis was detected by Annexin V-FITC / Propidium iodide( PI) double staining using flow cytometry. The expression of apoptotic and autophagy associated proteins as LC3,P62,Caspase-9,Caspase-3,the Caspase substrate poly( ADP-ribose) polymerase( PARP),Bcl-2 proteins family such as Bax,Bcl-2,PI3 k /Akt signaling pathway numbers such as Akt,p-Akt,m TOR,p-m TOR,P70s6 k and p-P70s6 k were detected by Western blotting. Results: Paris Polyphylla monomer pp-10 inhibited the proliferation of BGC-823 cells significantly in dose- and time- dependent manner( P 0. 05). Cell clone formation inhibition assay demonstrated that cell clones decreased with the increase of drug concentrations. Apoptotic morphology such as cell shrinkage,nuclear condensation,nuclear fragmentation,chromatin condensation were also observed by staining with Hoechst33342 under fluorescence microscope. Flow cytometry analysis showed an increase of the percentage of apoptotic cells in a dose-dependent manner treated with pp-10 for 24 h. Active bands of mitochondria associated apoptotic signaling pathway proteins such as Caspase-3,Caspase-9 and PARP could be seen with western blotting analysis after BGC-823 cells were incubated with different concentrations of pp-10 for24 h. In addition,pp-10 upregulated the expression of Bax,a pro-apoptotic protein,down-regulated the expression of Bcl-2 protein,an anti-apoptotic protein,up-regulated the expression of LC3,down-regulated the expression of P62 protein,and also down-regulated PI3 K / Akt signal pathway as decreasing the phosphorylation of p-m Tor,p-p70S6 K,downstream of Akt. Conclusions: pp-10 inhibits cell proliferation and induces the apoptosis and autophagy of human Gastric carcinoma BGC-823 cells by activation of Bax,down-regulation of Bcl-2 and reduction of PI3 K / Akt signaling pathway." @default.
- W2374738363 created "2016-06-24" @default.
- W2374738363 creator A5032592854 @default.
- W2374738363 date "2015-01-01" @default.
- W2374738363 modified "2023-09-25" @default.
- W2374738363 title "Pp-10,a Monomer Purified from Paris polyphylla,Induces Apoptosis and Autophagy of Human Gastric Carcinoma BGC-823 Cells by Inhibiting PI3K / Akt Signaling Pathway" @default.
- W2374738363 hasPublicationYear "2015" @default.
- W2374738363 type Work @default.
- W2374738363 sameAs 2374738363 @default.
- W2374738363 citedByCount "0" @default.
- W2374738363 crossrefType "journal-article" @default.
- W2374738363 hasAuthorship W2374738363A5032592854 @default.
- W2374738363 hasConcept C104317684 @default.
- W2374738363 hasConcept C153911025 @default.
- W2374738363 hasConcept C182979987 @default.
- W2374738363 hasConcept C185592680 @default.
- W2374738363 hasConcept C18903297 @default.
- W2374738363 hasConcept C190283241 @default.
- W2374738363 hasConcept C191015642 @default.
- W2374738363 hasConcept C2775934118 @default.
- W2374738363 hasConcept C2777527632 @default.
- W2374738363 hasConcept C31573885 @default.
- W2374738363 hasConcept C553184892 @default.
- W2374738363 hasConcept C55493867 @default.
- W2374738363 hasConcept C62112901 @default.
- W2374738363 hasConcept C75217442 @default.
- W2374738363 hasConcept C82381507 @default.
- W2374738363 hasConcept C86554907 @default.
- W2374738363 hasConcept C86803240 @default.
- W2374738363 hasConcept C88634738 @default.
- W2374738363 hasConcept C95444343 @default.
- W2374738363 hasConceptScore W2374738363C104317684 @default.
- W2374738363 hasConceptScore W2374738363C153911025 @default.
- W2374738363 hasConceptScore W2374738363C182979987 @default.
- W2374738363 hasConceptScore W2374738363C185592680 @default.
- W2374738363 hasConceptScore W2374738363C18903297 @default.
- W2374738363 hasConceptScore W2374738363C190283241 @default.
- W2374738363 hasConceptScore W2374738363C191015642 @default.
- W2374738363 hasConceptScore W2374738363C2775934118 @default.
- W2374738363 hasConceptScore W2374738363C2777527632 @default.
- W2374738363 hasConceptScore W2374738363C31573885 @default.
- W2374738363 hasConceptScore W2374738363C553184892 @default.
- W2374738363 hasConceptScore W2374738363C55493867 @default.
- W2374738363 hasConceptScore W2374738363C62112901 @default.
- W2374738363 hasConceptScore W2374738363C75217442 @default.
- W2374738363 hasConceptScore W2374738363C82381507 @default.
- W2374738363 hasConceptScore W2374738363C86554907 @default.
- W2374738363 hasConceptScore W2374738363C86803240 @default.
- W2374738363 hasConceptScore W2374738363C88634738 @default.
- W2374738363 hasConceptScore W2374738363C95444343 @default.
- W2374738363 hasLocation W23747383631 @default.
- W2374738363 hasOpenAccess W2374738363 @default.
- W2374738363 hasPrimaryLocation W23747383631 @default.
- W2374738363 hasRelatedWork W1930282135 @default.
- W2374738363 hasRelatedWork W2352900085 @default.
- W2374738363 hasRelatedWork W2355707334 @default.
- W2374738363 hasRelatedWork W2359915028 @default.
- W2374738363 hasRelatedWork W2361394353 @default.
- W2374738363 hasRelatedWork W2370786453 @default.
- W2374738363 hasRelatedWork W2371139612 @default.
- W2374738363 hasRelatedWork W2373490020 @default.
- W2374738363 hasRelatedWork W2379597309 @default.
- W2374738363 hasRelatedWork W2382325980 @default.
- W2374738363 hasRelatedWork W2385739811 @default.
- W2374738363 hasRelatedWork W2388501386 @default.
- W2374738363 hasRelatedWork W2405909062 @default.
- W2374738363 hasRelatedWork W2411193031 @default.
- W2374738363 hasRelatedWork W2474253747 @default.
- W2374738363 hasRelatedWork W2789936499 @default.
- W2374738363 hasRelatedWork W2978978324 @default.
- W2374738363 hasRelatedWork W2985373975 @default.
- W2374738363 hasRelatedWork W3029321630 @default.
- W2374738363 hasRelatedWork W3141496532 @default.
- W2374738363 isParatext "false" @default.
- W2374738363 isRetracted "false" @default.
- W2374738363 magId "2374738363" @default.
- W2374738363 workType "article" @default.