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- W2374740125 abstract "In this study,the total RNA was isolated from Guangxi Bama mini-pig ovary tissue with RNAwatson extraction.The complementary DNA(cDNA) encoding follistatin protein was ampilified by the reverse transcription polymerase chain reaction(RT-PCR) method and a pair of differential primers.The amplified cDNA fragment was inserted into pMD18-T vector.The recombined vectors were verified through the sequence survey.The results showed that the cloned follistatin cDNA was highly homologous with the reported nucleotide encoding swine follistatin in GenBank(99.4%).Meanwhile,the identified follistatin cDNA was inserted in the multiple cloning sites of plasmid pET 32a+ to construct prokaryotic expression vector,the latter was transformed into the E coli BL21 induced by IPTG. Then a fusion protein was obtained(about 58.4 ku) in SDS-PAGE and Western blotting. These results showed that the follistatin cDNA was expressed in prokaryotic cells." @default.
- W2374740125 created "2016-06-24" @default.
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- W2374740125 date "2007-01-01" @default.
- W2374740125 modified "2023-09-24" @default.
- W2374740125 title "Cloning and Prokaryotic Expression of Follistatin Gene from the Guangxi Bama Mini-pig" @default.
- W2374740125 hasPublicationYear "2007" @default.
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