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- W2374744513 abstract "Objective:To induce the chondrogenesis of bone marrow derived mesenchymal stem cell in rotating wall vessel (RWV) bioreactor and make tissue engineering cartilage in vitro.Methods:Bone marrow derived mesenchymal stem cells (bMSC) from adult rabbits were separated by gradient density centrifugation and amplified in plates,and were mixed in fibrin sealant gel at final concentration of 2×10 7/ml and then incubated in RWV bioreactor at 37 ℃,5%CO 2.The parallel static culture was performed as control group.Gross appearance,histological section,immunohistochemistry and cell vitality were investigated after 5 weeks.Results:RWV bioreactor could preserve better gross appearance of compounded material,the sections revealed that Toluidine blue and Ⅱ collagen stain were obviously positive,and the cell vitality of culture in the RWV was higher than the control.Conclusion:RWV bioreactor can efficiently improve the cell activity of cartilage,and it is a preferable in vitro method for cartilage differentiation of three dimension biomaterial." @default.
- W2374744513 created "2016-06-24" @default.
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- W2374744513 date "2004-01-01" @default.
- W2374744513 modified "2023-09-25" @default.
- W2374744513 title "In vitro chondrogenesis of tissue engineered biomaterial in rotating wall vessel bioreactor" @default.
- W2374744513 hasPublicationYear "2004" @default.
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