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- W2374774625 abstract "Objective:To prepare the Ni 2+ affinity gel and to test its ability to purify His 6_tag proteins.Methods:In the strong basic condition, Sepharose 4B was activated by epichlorohydrin and conjugated the arms of IDA (iminodiacetate). The arms of IDA were chelated with Ni 2+ and the Ni 2+ affinity gel was formed. Under denaturing and native conditions, the inclusion bodies of PARP domain of human tankyrase and soluble protein of human TRF1 were purified,respectively. Results:The purified proteins showed a single band on SDS_PAGE and were identified by Western_blot.Conclusions:The self_made Ni 2+ affinity gel has the same ability for purifying His 6_tag proteins as commercial gel and is much cheaper than the latter." @default.
- W2374774625 created "2016-06-24" @default.
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- W2374774625 date "2003-01-01" @default.
- W2374774625 modified "2023-09-25" @default.
- W2374774625 title "Preparation of Ni~(2+) affinity gel and purification of His_6-tag recombinant proteins" @default.
- W2374774625 hasPublicationYear "2003" @default.
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