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- W2374795570 abstract "An anodic stripping immunoassay based on gold label has been developed.The immunoreaction is performed in a polystyrene microwell using the sandwich format.Rabbit anti-human immunoglobulin G(IgG) antibodies were adsorbed passively on the walls of a polystyrene microwell.The IgG analyte was first captured by the rabbit anti-human antibody and then sandwiched by a colloidal gold-labeled goat anti-human IgG antibody.The subsequent immunocomplex interaction between the colloidal gold-labeled goat anti-human IgG antibody and the colloidal gold-labeled rabbit anti-goat IgG secondary antibody results in the adsorption of a large amount of colloidal gold onto the walls of a polystyrene microwell,which,after gold metal dissolution in an appropriate solution,was determined by anodic stripping voltammetry(ASV) at a carbon-paste electrode.The influence of some immunoassay conditions upon the anodic stripping peak current was examined and optimized.The anodic stripping peak current depended linearly on the logarithm of IgG concentration over the range of 1.1 ng/mL to 1 143 ng/mL and a detection limit as low as 1 ng/mL was achieved.The anodic stripping immunoassay was applied to the determination of IgG concentration in human serum with satisfactory results." @default.
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- W2374795570 date "2006-01-01" @default.
- W2374795570 modified "2023-09-23" @default.
- W2374795570 title "Anodic Stripping Immunoassay Based on Gold Label for Determination of Immunoglobulin G" @default.
- W2374795570 hasPublicationYear "2006" @default.
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