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- W2374842906 abstract "Objective: To clone mice macrophage migration inhibitory factor (MIF)cDNA and to construct mice MIF procaryotic exprssion vector. Methods: RT PCR was used to amplify the target gene with total RNA from mice spleen as template and specific oligo nucleotides as primers of MIF. The gene was inserted into pMD18 T and then sequenced.Then the procaryotic expression vector pQE31 MIF containing MIF cDNA was constructed and identified. Results:PCR amplified DNA fragment was cloned into pMD18 T and clonal recombinant of pMD18 MIF and procaryoticexpression vector pQE31 MIF was successfully constructed. Conclusion: Clonal recombinant of pMD18 MIF is obtained and MIF cDNA sequence is the same with that reported previously and procaryotic expression vector pQE31 MIF was successfully constructed." @default.
- W2374842906 created "2016-06-24" @default.
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- W2374842906 date "2002-01-01" @default.
- W2374842906 modified "2023-09-25" @default.
- W2374842906 title "Construction of mice migration inhibitory factor (MIF) procaryotic expression vector pQE31-MIF" @default.
- W2374842906 hasPublicationYear "2002" @default.
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