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- W2374869908 abstract "Objective To observe the dynamic progress of apoptosis and necrosis of pneumocytes during donor lung preservation, we tried to build a model to accurately assess graft function. Methods Experiments were performed with 42 male inbred (250 to 350 g) Sprague-Dawley rats, which were divided into 7 groups based on preservation times (0,2,4,6,12,18,24 h). The heart-lung block was then removed and stored in iced low potassium dextran(LPD) solution at 4 ℃ for different times. All lungs were flushed with 20 mL of a 500 mmol/L trypan blue solution through the main pulmonary artery, and then with 20 mL of 0.9% normal saline and 10 mL of 4% paraformaldehyde. The formalin-fixed lung tissues were embedded in paraffin and cut into 4-μm-thick tissue slices. We observed trypan blue staining with light microscopy. Detection of apoptosis through in situ TUNEL was undertaken, using in situ cell death detection kit according to the manufacturer’s instructions. Hoechst 33258 was used to show all the nucleated cells. Under fluorescent microscopy, cell counts were done by two researchers in a blinded fashion. Results The number of cell death increased and the type of the cell death changed by prolonged cold preservation time of the donor lungs. After cold ischemic storage, minimal cell death was seen in the 4 h groups. Significant cell death was detected only in lungs after 18 h of cold storage as determined through trypan blue-positive staining. On the contratry, apopotic cell begins in the 2 h groups, reach about 9% of all the nucleated cells in 12 h groups, and diminished in 18 h groups. Conclusions The study demonstrates a significant association among cold preservation time, extent and mode of cell death, suggesting new potential strategies to accurately assess graft function." @default.
- W2374869908 created "2016-06-24" @default.
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- W2374869908 date "2008-01-01" @default.
- W2374869908 modified "2023-09-26" @default.
- W2374869908 title "The dynamic progress of cell death during donor lung preservation" @default.
- W2374869908 hasPublicationYear "2008" @default.
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