Matches in SemOpenAlex for { <https://semopenalex.org/work/W2374876231> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2374876231 startingPage "1613" @default.
- W2374876231 abstract "In order to explore the effects of 35C > T and 682A > G mutations on the activity of alpha-(1,2) fucosyltransferase, the coding region of fut1 gene was amplified by polymerase chain reaction (PCR) from genomic DNA. PCR product was ligated into expression vector using TOPO TA cloning kit to obtain the recombinant plasmids. The recombinant plasmids were transfected into COS-7 cells by liposome method. After screening by using G418, H antigen expression on the COS-7 was tested by flow cytometry and fut1 mRNA was detected by real-time PCR. The results indicated that three kinds of recombinant plasmids pcDNA3.1/V5-His-wild (35C + 682A), pcDNA3.1/V5-His-35T and pcDNA3.1/V5-His-35T-682G were successfully constructed. After transfection, the H antigen expressed on membrane of COS-7 cells at the second day, with the maximum level of expression at the fourth day. When compared with pcDNA3.1/V5-His-wild transfected cells, the H antigen expression level of the 35T and 682G + 35T recombinant plasmids in the transfected cells was 52.7% and 13.3% on the fourth day, respectively. Although the level of fut1 mRNA decreased with prolonging of time, the mRNA expressed on the pcDNA3.1/V5-His-35T-682G transfected cells reached to 14% of the wild plasmids on the first day. It is concluded that 682A > G mutation obviously reduces the activity of alpha-(1,2) fucosyltransferase, while 35C > T mutation leads to partial reduction of H antigen in vitro expression." @default.
- W2374876231 created "2016-06-24" @default.
- W2374876231 creator A5005731833 @default.
- W2374876231 creator A5014890333 @default.
- W2374876231 creator A5018641343 @default.
- W2374876231 creator A5039457832 @default.
- W2374876231 creator A5043608589 @default.
- W2374876231 creator A5061546439 @default.
- W2374876231 creator A5064975153 @default.
- W2374876231 creator A5038106366 @default.
- W2374876231 date "2010-12-01" @default.
- W2374876231 modified "2023-09-22" @default.
- W2374876231 title "[In vitro expression activity of α-(1,2) fucosyltransferase with 35C > T and 682A > G mutations]." @default.
- W2374876231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21176381" @default.
- W2374876231 hasPublicationYear "2010" @default.
- W2374876231 type Work @default.
- W2374876231 sameAs 2374876231 @default.
- W2374876231 citedByCount "0" @default.
- W2374876231 crossrefType "journal-article" @default.
- W2374876231 hasAuthorship W2374876231A5005731833 @default.
- W2374876231 hasAuthorship W2374876231A5014890333 @default.
- W2374876231 hasAuthorship W2374876231A5018641343 @default.
- W2374876231 hasAuthorship W2374876231A5038106366 @default.
- W2374876231 hasAuthorship W2374876231A5039457832 @default.
- W2374876231 hasAuthorship W2374876231A5043608589 @default.
- W2374876231 hasAuthorship W2374876231A5061546439 @default.
- W2374876231 hasAuthorship W2374876231A5064975153 @default.
- W2374876231 hasConcept C104317684 @default.
- W2374876231 hasConcept C147483822 @default.
- W2374876231 hasConcept C153911025 @default.
- W2374876231 hasConcept C22744801 @default.
- W2374876231 hasConcept C2777762822 @default.
- W2374876231 hasConcept C33161422 @default.
- W2374876231 hasConcept C40767141 @default.
- W2374876231 hasConcept C54009773 @default.
- W2374876231 hasConcept C54355233 @default.
- W2374876231 hasConcept C553184892 @default.
- W2374876231 hasConcept C86803240 @default.
- W2374876231 hasConceptScore W2374876231C104317684 @default.
- W2374876231 hasConceptScore W2374876231C147483822 @default.
- W2374876231 hasConceptScore W2374876231C153911025 @default.
- W2374876231 hasConceptScore W2374876231C22744801 @default.
- W2374876231 hasConceptScore W2374876231C2777762822 @default.
- W2374876231 hasConceptScore W2374876231C33161422 @default.
- W2374876231 hasConceptScore W2374876231C40767141 @default.
- W2374876231 hasConceptScore W2374876231C54009773 @default.
- W2374876231 hasConceptScore W2374876231C54355233 @default.
- W2374876231 hasConceptScore W2374876231C553184892 @default.
- W2374876231 hasConceptScore W2374876231C86803240 @default.
- W2374876231 hasIssue "6" @default.
- W2374876231 hasLocation W23748762311 @default.
- W2374876231 hasLocation W23748762312 @default.
- W2374876231 hasOpenAccess W2374876231 @default.
- W2374876231 hasPrimaryLocation W23748762311 @default.
- W2374876231 hasRelatedWork W2058413229 @default.
- W2374876231 hasRelatedWork W2347849612 @default.
- W2374876231 hasRelatedWork W2355481742 @default.
- W2374876231 hasRelatedWork W2356234752 @default.
- W2374876231 hasRelatedWork W2361376727 @default.
- W2374876231 hasRelatedWork W2361639863 @default.
- W2374876231 hasRelatedWork W2362492361 @default.
- W2374876231 hasRelatedWork W2363280552 @default.
- W2374876231 hasRelatedWork W2365457430 @default.
- W2374876231 hasRelatedWork W2370183225 @default.
- W2374876231 hasRelatedWork W2375301618 @default.
- W2374876231 hasRelatedWork W2376147624 @default.
- W2374876231 hasRelatedWork W2376161078 @default.
- W2374876231 hasRelatedWork W2376869834 @default.
- W2374876231 hasRelatedWork W2380570708 @default.
- W2374876231 hasRelatedWork W2389360336 @default.
- W2374876231 hasRelatedWork W2409995530 @default.
- W2374876231 hasRelatedWork W2415998668 @default.
- W2374876231 hasRelatedWork W3030276197 @default.
- W2374876231 hasRelatedWork W3032312864 @default.
- W2374876231 hasVolume "18" @default.
- W2374876231 isParatext "false" @default.
- W2374876231 isRetracted "false" @default.
- W2374876231 magId "2374876231" @default.
- W2374876231 workType "article" @default.