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- W2374894887 abstract "Objective To study the effects of mouse embryonic fibroblast (MEF) feeder layers and the combination of MEF and collagen of rat tail on adherence of the cultured cells from Schistosoma japonicum (S. japonicum). Methods The 21 days schistosomula of S. japonicum were harvested by perfusion. Cell suspension was made by cold digestion and diluted to a concentration of 1 × 109 cells/L. Then, the cells were inoculated and cultured on the walls of culture flasks that had been previously smeared with collagen of rat tail or/and cultured MEF and were divided into collagen group, MEF group and collagen combined with MEF group, respectively. The cells cultured without collagen of rat tail and MEF was set up as control group. The number of the adherent cells was counted timely, while the rate of cell adherence was calculated. Results It was found that the rate of cell adherence increased gradually along with the extending of the culture times from 24 to 48 hours, but decreased after 48 hours. Analysis of variance showed that the rates of cell adherence had differences in different culture time (F = 149.22, 81.97, 232.86, 63.05, P 0.01).q-test analysis revealed that the rates of cell adherence in the same group had significant differences in different culture time(P 0.01). At the same culture time, analysis of variance showed that the rates of cell adherence had difference among the four groups (F24 = 44.65,P 0.01 ;F48 = 1 071.489,P 0.01 ;F72 = 4 208.35,P 0.01). q-test analysis revealed that there was a significant difference rate of cell adherence (P 0.01) except for that between MEF group and collagen group cultured for 24 h (q = 0.792, P 0.05). Conclusions MEF has obvious effects on the adherence of cultured cells of S. japonicum, and the combination of MEF and collagen of rat tail has synergetic effects on the adherence of cultured cells." @default.
- W2374894887 created "2016-06-24" @default.
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- W2374894887 date "2006-01-01" @default.
- W2374894887 modified "2023-09-26" @default.
- W2374894887 title "Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from Schistosoma japonicum" @default.
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