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- W2374920459 abstract "Dendrocalamopsis oldhami( Do) was used as the experimental material. By homology cloning,two specific fragments were isolated from Do genome DNA with specific primers,named Do P5CS1 and Do P5CS2. The length of sequence Do P5CS1 was 2 178 bp,containing 7 introns and 8 exons. The exons sequence was 976 bp,which coded 325 amino acids residue,the molecular weight and the isoelectric point of coded protein were 34. 787 4 k D and 5. 27,respectively. The coded protein contained an amino acid kinases domain in N terminal and an aldehyde dehydrogenase motif in C terminal. The length of Do P5CS2 was the same as the exons sequence of Do P5CS1,showing high identity( 99. 6%). But the introns were absent in the Do P5CS2,so it might be a pseudogene. The above results laid the foundation for isolating the full length of the P5 CS gene and its function analysis,and also for increasing stress resistance of Do or even other bamboo species by molecular biology methods." @default.
- W2374920459 created "2016-06-24" @default.
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- W2374920459 date "2015-01-01" @default.
- W2374920459 modified "2023-09-24" @default.
- W2374920459 title "Amplification and Sequence Analysis of P5CS in Dendrocalamopsis oldhami" @default.
- W2374920459 hasPublicationYear "2015" @default.
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