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- W2374920597 abstract "Aim To clone and sequence adhesin gene papG obtained from uropathogenic E.coli strain 132 (UPEC132).Methods papG of UPEC132 was amplified by PCR with primer pairs specific for two classes of papG with one of them for UPECJ96 whose F13 serotype of P pili contained classⅠpapG and the other for UPECIA2 characterized by F11 serotype of P pili including classⅡpapG.The recombinants constructed by the insertion of the amplified fragment from UPEC132 into the plasmid pUC18 were then identified and subjected to DNA sequencing.Results A single predicted fragment of about 1100 bp from UPEC132 was identified with the primer specific for classⅡpapG but not the primer specific for classⅠpapG.Sequence analysis of the recombinant plasmids(pGC39 and pGC103) carrying such a product of papG of UPEC132 (PapG132) showed that a 377 amino acid long polypeptide could be translated.UPEC132 and J96 shared only 45% homology of deduced amino acid sequence, but the homology of deduced amino acid sequence between UPEC132 and IA2 was 98.6%.There were four missense mutations in the PapG of UPEC132 with two of them involved in the receptor binding domain and the other two in the PapD PapG interaction domain, as compared to IA2.Seven synonymous mutations were also shown, as well as a deleted codon at +3 site occurred, causing one amino acid residue shorter in PapG132 protein than in PapGIA2 equivalent. Conclusion The PapG adhesin from UPEC132 differs from extensively from the corresponding protein from J96, although both strains expressed the same serotype of P pili.Thus, the structure of fimbriae of UPEC132 consists of class II PapG variant in conjunction with the F13 PapA protein." @default.
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- W2374920597 date "2002-01-01" @default.
- W2374920597 modified "2023-09-23" @default.
- W2374920597 title "Cloning and sequencing of adhesin gene papG derived from uropathogenic Escherichia coli" @default.
- W2374920597 hasPublicationYear "2002" @default.
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