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- W2374929905 abstract "Objective To purify the hepatitis B virus(HBV) preS2(epi)S protein expressed in Pichia pastoris and study its immune effect.Methods Induce recombinant Pichia pastoris containing preS2(epi)S gene with methanol,and purify the expressed product by sucrose zonal centrifugation,Butyl-S-Sepharose 4FF hydrophobic and DEAE-Sepharose Fast Flow ion exchange chromatography and identify by SDS-PAGE and Western blot.Prepare vaccine with the purified protein and perform potency test in mice.Determine the serum antibody concentration of mice by RIA and calculate ED50 and relative potency.Test the antibody against preS2(epi)S by ELISA.Results The purified preS2(epi)S protein with a relative molecular mass of about 27 000 reacted with specific antibody.The ED50 of 2 batches of vaccine prepared with the purified protein were 0.35 and 0.48 μg/ml respectively,while that of reference vaccine was 0.52 μg/ml.The mean serum antibody concentrations induced by the 2 batches of vaccine were 808 and 784 mIU/ml respectively,while that by reference vaccine was 312 mIU/ml.The relative potencies of the 2 batches of vaccine were 1.5 and 1.1 respectively.Both the 2 batches of vaccine induced antibody against preS2 in immunized mice.Conclusion The purification method was practical and effective,and the purified preS2(epi)S protein showed good immunogenicity compared with S protein." @default.
- W2374929905 created "2016-06-24" @default.
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- W2374929905 date "2007-01-01" @default.
- W2374929905 modified "2023-09-22" @default.
- W2374929905 title "Purification and Immune Effect of Hepatitis B Virus preS2(epi)S Protein Expressed in Pichia pastoris" @default.
- W2374929905 hasPublicationYear "2007" @default.
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