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- W2374974566 abstract "Objective To study the effects of PPARγ on differentiation of mouse marrow stroma cells ( MSCs) into cardiomyogenesis. Methods Primary mouse MSCs were collected to culture. The expression vector pEGFP-N1-PPARγ2 was constructed and transfected into MSCs by liposome. Transfected MSCs were screened by G418. RT-PCR was used to measure the expression of mRNA. Protein expression was measured by Western blot. Results Undifferentiated MSCs didn't express PPARγ mRNA but GATA4. MEF2C and Nkx2. 5 did. After transfected with pEGFP-N1-PPARγ2, MSC differentiated into adipocytes but not cardiomyogenesis, and the expressions of GATA4.MEF2C.Nkx2. 5 mRNA were inhibited significantly. Conclusions PPARγcould inhibit the expressions of GATA4.MEF2C,Nkx2. 5 mRNA in MSCs repress MSCs differentiating into cardiomyogenesis and promote them differentiating into adipocytes." @default.
- W2374974566 created "2016-06-24" @default.
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- W2374974566 date "2004-01-01" @default.
- W2374974566 modified "2023-09-24" @default.
- W2374974566 title "The effects of PPARγ transfection on differentiation of mouse marrow stroma cells into cardiomyogenesis" @default.
- W2374974566 hasPublicationYear "2004" @default.
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