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- W2374977328 abstract "Objective:To prepare monoclonal antibody (McAb) of human breast cancer associated antigen and to lay a foundation for further study of drug guided diagnosis and treatment.Methods:Immunization of Balb/c mice with of human breast cancer cell line ZR75,the study applied lymphocytes hybridoma techniques in McAb preparation and used immunofluorescence、immunohistochemistry (IHC) and Western blotting in the characterization of antigen recognized by McAb BM109.Results:One hybridoma cell line (BM109),which secretes monoclonal antibody against breast carcinoma,was established.McAb BM109 was found to be of the IgG 1 isotope by double diffusion.The antigen recognized by BM109 was a protein with a molecular weight of 37KD and located on ZR75 cell membrane.The reactivity of BM109 to breast cancer and several other kinds of tumors was examined with IHC,the results showed that the BM109 reacted strongly to most breast carcinoma and did not react to normal breast tissues and other kinds of tumors,such as gastric cancer、esophagus cancer、lung cancer and other three tumors.Conclusion:The hybridoma cell line BM109 stably secreting antibody reacted specifically with breast carcinoma was obtained and the McAb BM109 might be used in basic and clinical researches of breast cancer with a bright prospect. [" @default.
- W2374977328 created "2016-06-24" @default.
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- W2374977328 date "2001-01-01" @default.
- W2374977328 modified "2023-09-23" @default.
- W2374977328 title "Preparation of Monoclonal Antibody Against Human Breast Cancer and its Application in Tumor Research" @default.
- W2374977328 hasPublicationYear "2001" @default.
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