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- W2375008238 abstract "Objective To develop a rapid,high-throughput screening method of a multiplex PCR-based reverse line blot(mPCR/RLB) array to detect S.pneumoniae,H.influenzae and N.meningitides.Methods Highly validated specific primers and probes were used to diagnostic regions unique to each pathogen.Primers,dNTP,and probes in various concentrations were detected to determine the optimal conditions.Results The limit of detection of mPCR/RLB array was 6.2×10-1ng/μl for S.pneumoniae,with 8.5×10-8ng/μl genome DNA for H.influenzae,and 8.3×10-8ng/μl for N.meningitides.Conclutions mPCR/RLB array can detect three pathogen correctly,and it is valuable for high-throughput screening pathogens of clinical samples of bacterial meningitis." @default.
- W2375008238 created "2016-06-24" @default.
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- W2375008238 date "2011-01-01" @default.
- W2375008238 modified "2023-09-27" @default.
- W2375008238 title "Study of multiplex PCR-based reverse line blot array for pathogen detection in bacterial meningitis" @default.
- W2375008238 hasPublicationYear "2011" @default.
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