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- W2375064692 abstract "Objective To improve superovulation and establish a method of embryo freezing for rats.Methods Different doses of PMSG-HCG (15 IU,20 IU,25 IU/rat) were injected (i.p.) into three groups(Group A,B,C) of 5 weeks SD rats,and each treated female was coupled with fertile male,the effect of each dose was compared to determine the best dose for superovulation.Two-cell was collected by clysising fimbriae tubae.The embryos were divided into 2 groups.Embryos in group 1 were frozen for 1 minute,and embryos in group 2 for 5 minutes.All the embryos of rats were frozen according to the standard method for mouse embryos frozen.The embryos were resuscitated,and the rate of resuscita-tion were compared to assess the two method.Results The average number of zygotes obtained from each rat in group A,B,C was 13±12.7,31±14.2,and 41±25.4.There were significant differences among the groups (P0.05).The resuscitation rate of embryos in group 1 is 88.3%,and 84.9% in group 2.Of 152 rat embryos were frozen for 1 minute,139 embryos were successfully resuscitated,and cultured for 20 minutes.The normal cultured 105 embryos were transplanted into pseudopreg-nant rats,and 39 offspring were obtained.Conclusion The method for rat superovulation has been successfully improved,and a method of embryo freezing for rats has been established." @default.
- W2375064692 created "2016-06-24" @default.
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- W2375064692 date "2008-01-01" @default.
- W2375064692 modified "2023-09-23" @default.
- W2375064692 title "Comparative Study on Superovulation and Embryo Freezing in SD Rats" @default.
- W2375064692 hasPublicationYear "2008" @default.
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