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- W2375083592 abstract "pMSCV containing GFP and neo-gene was transfected into the cattle fetal auris fibroblast cells by lipofection or electrofusion was the donor.The maturation of bovine oocyte in vitro was receptor.The embryo cloning was constructed by the donor and receptor.Under different conditions,such as adding epidermal growth factor(EGF)to the culture medium improved the nuclear maturation and transgenic embryos in vitro culture,donor cells gene-transfection method,conducted to investigate the effect of three in vitro culture systems on the development of bovine embryos and to observe the development of bovine transgenic embryos in vitro in the optimal culture system will be studied.To examine the effects of EGF on the nuclear and cytoplasm maturation of cattle oocytes in vitro culture,the best concentration of EGF was 30 ng/mL,then the oocytes were activated by 6-dimethylaminopurine combined with ethanol,examining the cleavage rate,blastocyst development rate.The cleavage rate and blastocyst development rate adding 25 ng/mL EGF to medium group were significantly higher than those in control group;and its effect on cell proliferation after grafting.The different gene-transfection method was beneficial to transfer embryo.The rate of the integration was the blastocyst development rate of cattle oocytes significantly(P0.05);the highest mSOFaa+granulosa cell monolayer co-culture system." @default.
- W2375083592 created "2016-06-24" @default.
- W2375083592 creator A5058969844 @default.
- W2375083592 date "2009-01-01" @default.
- W2375083592 modified "2023-09-26" @default.
- W2375083592 title "Study on Transgenic GFP Gene Bovine Embryos Production by Nuclear Transfer" @default.
- W2375083592 hasPublicationYear "2009" @default.
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