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- W2375097752 abstract "Objective:To detect a better method to obtain purified and viable Langerhans cells from human epidermis and observe the effect of imiquimod on the mRNA expressions of cytokines from human epidermal Langerhans cells.Methods:Density gradient centrifugation and magnetic cell sorting were used simultaneously to separate and purify the Langerhans cells from human epidermis.The separated cells were specially marked by monoclonal mouse-anti-human CD1a antibody conjugated to FITC.The purification rate was analyzed by flow cytometric fluorography analysis and the cell viability was observed by 0.4% trypan blue staining.Langerhans cells were divided into two groups--experimental group and control group.The solution of 5 μg/ml imiquimod was added to the experimental group and the culture medium for the control group.The total RNA was harvested 4 h after application.RT-PCR was conducted to detect the mRNA expressions of TNF-α,IL-1β and IL-6 in each group.Results:The purification rate of Langerhans cells was 84.48% and the viability of Langerhans cells was more than 95%.Expression levels of TNF-α,IL-1β and IL-6 mRNA of Langerhans cells 4 h after 5 μg/ml imiquimod application were statistically higher than the controls(P0.05).Conclusion:Combining the techiques of density gradient centrifugation and magnetic cell sorting is highly effective to obtain the better purificated and more active Langerhans cells from human skin.And this method is easy to manipulate under sterile conditions.The imiquimod could increase the mRNA expression of TNF-α,IL-1β and IL-6 from sorted Langhans cells." @default.
- W2375097752 created "2016-06-24" @default.
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- W2375097752 date "2007-01-01" @default.
- W2375097752 modified "2023-09-24" @default.
- W2375097752 title "The reaction of purified human epidermal langerhans cells to imiquimod" @default.
- W2375097752 hasPublicationYear "2007" @default.
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