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- W2375101275 abstract "【Objective】To isolate and prepare c-kit-positive cardiac stem cells for the study of autos cardiac regeneration therapy for cardiac infarct.【Methods】Adult rat heart was digested with Ca2+-Mg2+-free buffer containing collagenase and trypsin to obtain suspended monocells.The suspended monocells were treated with anti-c-kit primary antibody and secondary antibody conjugated with magnetic microbeads,and then the c-kit + cells were separated by magnetic cell sorting equipment.Under the conditions of differentiation medium,the isolated c-kit-positive cells were induced to differentiate.【Results】Confocal immunofluorescent analysis showed that c-kit positive cells were more than 90%.Real-time PCR and confocal immunofluorescent analysis results indicated that the cardiomyocytes derived from c-kit+ cardiac stem cells expressed cardiac specific transcription factors gata4 and cardiac specific contractile proteinsα-sarcomeric actin and Troponin I; the vascular smooth muscle and endothelial cells derived from c-kit+ cardiac stem cells expressed SM22α,α-SMA,KDR and CD31【.Conclusion】The isolated c-kit-positive cardiac stem cells are multipotent.It could be induced into three different cardiogenic cell lineages: cardiac myocytes,vascular smooth muscle,and vascular endothelial cells." @default.
- W2375101275 created "2016-06-24" @default.
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- W2375101275 date "2013-01-01" @default.
- W2375101275 modified "2023-09-22" @default.
- W2375101275 title "Study on the preparation and multipotent differentiation of c-kit-positive cardiac stem cells" @default.
- W2375101275 hasPublicationYear "2013" @default.
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