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- W2375111191 abstract "Using the Aspergillus Kawachii genetic engineering strain TR12 and Aspergillus niger 34309 as the original strains, two parent protoplasts were obtained respectively in cellulase solution or mixed solution with cellulase and lysozyme. Based of the study on the impact to the protoplasts under different conditions of culture time, culture method, enzymolysis time and prescription of enzymolysis solution, protoplasts fusion was done in the PEG solution. Then mutation breeding was made with ultraviolet ray radiation. Through comparing its colony appearance, color and configuration with TR12 and 34309, while carried through isolation culture and shakerflask culture, finally screen a better strain. The enzymic activities of acid stable α-amylase and glucoamylase reached 91.2?u/ml and 3216.2?u/ml, respectively. " @default.
- W2375111191 created "2016-06-24" @default.
- W2375111191 creator A5048537346 @default.
- W2375111191 date "2003-01-01" @default.
- W2375111191 modified "2023-09-25" @default.
- W2375111191 title "Screening of Higher Enzyme Activity Strain with Protoplast Fusion and Mutagenisis" @default.
- W2375111191 hasPublicationYear "2003" @default.
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