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- W2375143923 abstract "Objective To observe the expression of microRNA-199a(miR-199a) and its regulation on gene expression of A549 cells in mice with acute lung injury induced by lipopolysaccharide(LPS).Methods The animal model was established by intratracheal injection of LPS of a dose of 10mg/kg body weight into the lung of the mice.The miR-199a expression was determined by Northern blot.MicroRNA over-expression adenovirus was constructed and used to transfect A549 cells.The gene expression modulation effect of the miR-199a over-expression was tested using mRNA microarray.Results In mice with LPS-induced acute lung injury,the miR-199a expression was significantly down-regulated.MiR-199a could enhance the expression of metabolism and cell cycle related genes.Conclusion MiR-199a is down-expressed in mice with LPS-induced acute lung injury.MiR-199a over-expression can promote the expression of metabolism and cell cycle related genes." @default.
- W2375143923 created "2016-06-24" @default.
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- W2375143923 date "2011-01-01" @default.
- W2375143923 modified "2023-09-23" @default.
- W2375143923 title "MicroRNA-199a Expression and Regulation on Gene Expression in Mice with Lipopolysaccharide-induced Acute Lung Injury" @default.
- W2375143923 hasPublicationYear "2011" @default.
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