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- W2375149336 abstract "Objective:To establish a method for the determination of 2 alkaloids in root of Dactylicapnos scandens by RP-HPLC.Method:A Luna C18100A(4.6×250mm,5μm)was used with gradient elution the mobile phase of triethylamine-0.2% phosphoric acid(0.1∶35)(A)and methanol-acetonitrile(14∶1)(B).The flow rate was 1.0mL·min-1.The detection wavelength was 285nm with injection volume of 20μL and the column temperature was ℃.Results:The calibration curves was linear in the range of 0.065 mg/ml~0.195mg/ml for isocorydine,the regression equation being Y=19974X+2.3524(r=0.9999),and the average recovery was 97.0% with RSD 1.6%;The calibration curves was linear in the range of 0.025 mg/ml~0.075mg/ml for protopine,the regression equation being Y=20923X-4.3441(r=0.9998),and the average recovery was 98.9% with RSD 1.8%.Conclusion:The method is simple,accurate and reproducible for quality control of root of Dactylicapnos scandens." @default.
- W2375149336 created "2016-06-24" @default.
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- W2375149336 date "2009-01-01" @default.
- W2375149336 modified "2023-09-23" @default.
- W2375149336 title "Simultaneous RP-HPLC Determination of 2 Alkaloids in Root of Dactylicapnos scandens" @default.
- W2375149336 hasPublicationYear "2009" @default.
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