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- W2375157159 abstract "Phosphate (Pi) is one of the key substrate in energy metabolism and biosynthesis of nucleic acids and membranes.It also plays an important role in photosynthesis,respiration,and regulation of a number of enzymes.Most of the areas of wheat growth in China face the problem of phosphate deficiency.Generating varieties that can grow well under the condition of phosphate starvation is an important way to solve this problem.What genes expression changed when wheat grew under the condition of phosphate starvation?How did they change?Solving these problems is very important for generating transgenic wheat varieties that can produce adequate yields in phosphate-deficiency soils.Phosphate transporters were one important family of these genes.Using the known ESTs BQ744323(5′),BJ287203(3′),BJ260111(5′) and BJ265811(3′),two phosphate transporter gene cDNAs TaPT8 and TaPHT2;1 were isolated from common wheat by RT-PCR.The two phosphate transporter genes can complement the phosphate-deficiency in the yeast mutants. TaPT8 belonged to Pht1 family. TaPHT2;1 is one member of Pht2 family.Using RQRT-PCR,the expression of TaPT8 could only be found in roots and was up-regulated by phosphate starvation. TaPHT2;1 mainly expressed in green tissues.The expression of TaPHT2;1 was down-regulated by phosphate starvation and darkness but up-regulated by light using RQRT-PCR. TaPHT2;1 may play a role during the process of transport phosphate from cytoplasm into chloroplast." @default.
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- W2375157159 date "2004-01-01" @default.
- W2375157159 modified "2023-09-22" @default.
- W2375157159 title "Isolation,function and expression analysis of two wheat phosphate transporter genes" @default.
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