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- W2375190133 abstract "Objective To investigate the construction of synaptotagmin XI(SytXI) plasmid and overexpression,and its impact on PC12 cells and the aggresome formation.Methods SytXI was obtained by using human fetal brain cDNA library as template and then ligated into pcDNA3.1myc-His(-)B plasmid.The recombinant plasmid was identified by endonuclease and DNA sequencing.After transfected into PC12 cells,Annexin-V-PI staining and immunofluorescence were used to define its impact on the cells.Results The SytXI gene was ligated correctly into the vector.Overexpression of SytXI led to cell necrosis,and increased the formation of α-synuclein and vimentin positive aggresomes.Conclusions The recombinant plasmid was constructed and successfully transfected into PC12 cells.The overexpression of SytXI was toxic to PC12 cells and increased the formation of aggresomes." @default.
- W2375190133 created "2016-06-24" @default.
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- W2375190133 date "2007-01-01" @default.
- W2375190133 modified "2023-09-28" @default.
- W2375190133 title "Effects of Synaptotagmin XI Plasmid Overexpression on PC12 Cells and the Aggresomes Formation" @default.
- W2375190133 hasPublicationYear "2007" @default.
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